ECHS1 antibody

Cat.#: 107245

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Product Information

  • Product Name
    ECHS1 antibody
  • Documents
  • Description
    ECHS1 Mouse Monoclonal antibody. Positive IP detected in HepG2 cells. Positive WB detected in HepG2 cells, human testis tissue, PC-3 cells. Positive IHC detected in human liver tissue, human prostate cancer tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 31 kDa
  • Tested applications
    ELISA, WB, IHC, IP, IF
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    ECHS1 antibody; Enoyl CoA hydratase 1 antibody; SCEH antibody
  • Isotype
    Mouse IgG2b
  • Preparation
    This antibody was obtained by immunization of ECHS1 recombinant protein (Accession Number: NM_004092). Purification method: Protein A purified.
  • Clonality
    Monoclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:500-1:5000

    IHC: 1:20-1:200

    IF: 1:20-1:200

  • Validations

    HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:107245(ECHS1 antibody) at dilution of 1:1000

    HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:107245(ECHS1 antibody) at dilution of 1:1000

    IP Result of anti-ECHS1 (IP:Catalog No:107245, 3ug; Detection:Catalog No:107245 1:1000) with HepG2 cells lysate 3600ug.

    IP Result of anti-ECHS1 (IP:Catalog No:107245, 3ug; Detection:Catalog No:107245 1:1000) with HepG2 cells lysate 3600ug.

    Immunohistochemistry of paraffin-embedded human liver tissue slide using Catalog No:107245(ECHS1 Antibody) at dilution of 1:50

    Immunohistochemistry of paraffin-embedded human liver tissue slide using Catalog No:107245(ECHS1 Antibody) at dilution of 1:50

    Immunofluorescent analysis of HepG2 cells using Catalog No:107245(ECHS1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)

    Immunofluorescent analysis of HepG2 cells using Catalog No:107245(ECHS1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)

  • Background
    Enoyl-coenzyme A hydratase (ECHS1) is a mitochondrial protein which catalyzes the hydration of 2-trans-enoyl-coenzyme A intermediates to L-3-hydroxyacyl-coenzyme A, playing key role in metabolism of fatty acids in mitochondria. ECHS1 is highly expressed in muscle, liver and fibroblasts. Altered expression of ECHS1 has been considered as a characteristic feature of mitochondria dysfunction. (23275097, 23235493)
  • References
    • Xu WD, Yang XY, Li DH. Up-regulation of fatty acid oxidation in the ligament as a contributing factor of ankylosing spondylitis: A comparative proteomic study. Journal of proteomics. 113:57-72. 2015.
    • Haack TB, Jackson CB, Murayama K. Deficiency of ECHS1 causes mitochondrial encephalopathy with cardiac involvement. Annals of clinical and translational neurology. 2(5):492-509. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"