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EAF1 antibody

Cat.#: 110161

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Product Information

  • Product Name
    EAF1 antibody
  • Documents
  • Description
    EAF1 Rabbit Polyclonal antibody. Positive IF detected in SH-SY5Y cells. Positive IP detected in HeLa cells. Positive WB detected in K-562 cells, HeLa cells, mouse brain tissue. Observed molecular weight by Western-blot: 43 kDa
  • Tested applications
    ELISA, WB, IP, IF
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    EAF1 antibody; ELL assOCiated factor 1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of EAF1 recombinant protein (Accession Number: NM_033083). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:200-1:2000

    IF: 1:20-1:200

  • Validations

    K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:110161(EAF1 antibody) at dilution of 1:500

    K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:110161(EAF1 antibody) at dilution of 1:500

    IP Result of anti-EAF1 (IP:Catalog No:110161, 4ug; Detection:Catalog No:110161 1:500) with HeLa cells lysate 1200ug.

    IP Result of anti-EAF1 (IP:Catalog No:110161, 4ug; Detection:Catalog No:110161 1:500) with HeLa cells lysate 1200ug.

    Immunofluorescent analysis of SH-SY5Y cells using Catalog No:110161(EAF1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of SH-SY5Y cells using Catalog No:110161(EAF1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    ELL associated factor 1 and ELL associated factor 2 (EAF1/2 factors) are reported to play important roles in tumor suppression and embryogenesis. The tumor suppressor EAF2 is regulated by androgen signaling and associated with prostate cancer. Eaf factors have been revealed to modulate mesoderm and neural patterning by inhibiting canonical Wnt signaling, and high activity of canonical Wnt/β-catenin is revealed in eafs morphants (PMID: 23364330). Catalog#13787-1-AP is a rabbit polyclonal antibody raised against the full-length of human EAF1.
  • References
    • Sobhian B, Laguette N, Yatim A. HIV-1 Tat assembles a multifunctional transcription elongation complex and stably associates with the 7SK snRNP. Molecular cell. 38(3):439-51. 2010.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"