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Product Name
E2F8 antibody
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Description
E2F8 Rabbit Polyclonal antibody. Positive IP detected in HEK-293 cells. Positive WB detected in HeLa cells, HEK-293 cells. Observed molecular weight by Western-blot: 82 kDa, 105 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
E2F 8 antibody; E2F transcription factor 8 antibody; E2F8 antibody; FLJ23311 antibody; Transcription factor E2F8 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of E2F8 recombinant protein (Accession Number: BC028244). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110157(E2F8 antibody) at dilution of 1:1000
IP Result of anti-E2F8 (IP:Catalog No:110157, 4ug; Detection:Catalog No:110157 1:500) with HEK-293 cells lysate 2800ug.
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Background
E2F8 is one E2F transcription factor that is essential for orchestrating expression of genes required for cell cycle progression, proliferation, apoptosis and differentiation. E2F8 shows a high degree of resemblance to E2F7 and shares the unique structure of E2F7 by having two distinct domains exhibiting a high degree of similarity to the DNA-binding domain of the E2F familyTogether with E2F7, they possess two DNA-binding domains that are predicted to interact with each other. E2F8 binds consensus E2F sites in a DP-independent manner and represses transcription of E2F-regulated promoters. Ectopic expression of E2F8 inhibits cellular proliferation.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"