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Product Name
DVL1 Polyclonal Antibody
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Description
Polyclonal antibody to DVL1
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
DVL1 antibody; DRS2 antibody; DVL antibody; DVL1L1 antibody; DVL1P1 antibody; dishevelled segment polarity protein 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 510-640 of human DVL1 (NP_004412.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - DVL1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using DVL1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.
Immunohistochemistry - DVL1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat testis using DVL1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - DVL1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse testis using DVL1 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - DVL1 Polyclonal Antibody
Immunofluorescence analysis of NIH/3T3 cells using DVL1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes. Required for LEF1 activation upon WNT1 and WNT3A signaling. DVL1 and PAK1 form a ternary complex with MUSK which is important for MUSK-dependent regulation of AChR clustering during the formation of the neuromuscular junction (NMJ).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"