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Product Name
DUSP8 Polyclonal Antibody
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Description
Polyclonal antibody to DUSP8
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Tested applications
WB
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Species reactivity
Human, Mouse, Rat
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Alternative names
DUSP8 antibody; C11orf81 antibody; HB5 antibody; HVH-5 antibody; HVH8 antibody; dual specificity phosphatase 8 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant protein of human DUSP8
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:200 - 1:1000
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Validations
Western blot - DUSP8 Polyclonal Antibody
Western blot analysis of extracts of various cell line, using DUSP8 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.
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Background
Has phosphatase activity with synthetic phosphatase substrates and negatively regulates mitogen-activated protein kinase activity, presumably by catalysing their dephosphorylation. Expected to display protein phosphatase activity toward phosphotyrosine, phosphoserine and phosphothreonine residues.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"