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Product Name
DUSP6 antibody
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Description
DUSP6 Rabbit Polyclonal antibody. Positive IHC detected in human stomach cancer tissue, human liver cancer tissue, human stomach tissue. Positive IF detected in HeLa cells. Positive WB detected in Transfected HEK-293 cells.
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Tested applications
ELISA, IHC, IF, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
dual specificity phosphatase 6 antibody; DUSP6 antibody; MAP kinase phosphatase 3 antibody; MKP 3 antibody; MKP3 antibody; PYST1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DUSP6 recombinant protein (Accession Number: NM_001946). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:110121(DUSP6 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human stomach cancer tissue slide using Catalog No:110121(DUSP6 Antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemistry of paraffin-embedded human stomach cancer tissue slide using Catalog No:110121(DUSP6 Antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HeLa cells using Catalog No:110121(DUSP6 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Dual specificity phosphatase 6 (DUSP6) is a member of the family of mitogen-activated protein kinase (MAPK) phosphatases, which dephosphorylate and inactivate MAPK (PMID: 9858808). The MAPK pathway is an important signal transduction system that serves cellular processes, such as proliferation, differentiation, migration, and apoptosis. DUSP6 resides at chromosome location 12q22-23, which is one of the candidate loci for susceptibility to bipolar disorder and which encodes a phosphatase selective for extracellular signal-regulated kinase (ERK) (PMID: 9205128). DUSP6 inactivates extracellular signal-regulated kinase (ERK), and can act in tumor suppressive pathways, whose expression is reduced in several different types of cancer. (PMID: 21680106).
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References
- Kang TH, Kim KT. VRK3-mediated inactivation of ERK signaling in adult and embryonic rodent tissues. Biochimica et biophysica acta. 1783(1):49-58. 2008.
- Jeong HS, Park KC, Kim DS. PP2A and DUSP6 are involved in sphingosylphosphorylcholine-induced hypopigmentation. Molecular and cellular biochemistry. 367(1-2):43-9. 2012.
- Yang B, Tan Y, Sun H, Fan J, Tang Z, Ji Y. Higher intratumor than peritumor expression of DUSP6/MKP-3 is associated with recurrence after curative resection of hepatocellular carcinoma. Chinese medical journal. 127(7):1211-7. 2014.
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