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Product Name
DUSP4 Polyclonal Antibody
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Description
Polyclonal antibody to DUSP4
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Tested applications
WB, IHC
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Species reactivity
Human, Monkey
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Alternative names
DUSP4 antibody; HVH2 antibody; MKP-2 antibody; MKP2 antibody; TYP antibody; dual specificity phosphatase 4 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-168 of human DUSP4 (NP_001385.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100 -
Validations
Western blot - DUSP4 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using DUSP4 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
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Background
Regulates mitogenic signal transduction by dephosphorylating both Thr and Tyr residues on MAP kinases ERK1 and ERK2.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"