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Product Name
DSPP Polyclonal Antibody
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Description
Polyclonal antibody to DSPP
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Tested applications
WB
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Species reactivity
Human, Mouse, Rat
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Alternative names
DSPP antibody; DFNA39 antibody; DGI1 antibody; DMP3 antibody; DPP antibody; DSP antibody; dentin sialophosphoprotein antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 16-146 of human DSPP (NP_055023.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - DSPP Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using DSPP antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
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Background
DSP may be an important factor in dentinogenesis. DPP may bind high amount of calcium and facilitate initial mineralization of dentin matrix collagen as well as regulate the size and shape of the crystals.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"