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Product Name
DPF2 antibody
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Description
DPF2 Rabbit Polyclonal antibody. Positive IP detected in Jurkat cells. Positive WB detected in Apoptosised HeLa cells, Jurkat cells, K-562 cells, Y79 cells. Positive IHC detected in human lung cancer tissue, human testis tissue. Positive IF detected in HepG2 cells, Hela cells. Observed molecular weight by Western-blot: 44-50 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
BAF45D antibody; BRG1 assOCiated factor 45D antibody; DPF2 antibody; Protein requiem antibody; REQ antibody; ubi d4 antibody; UBID4 antibody; Zinc finger protein ubi d4 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DPF2 recombinant protein (Accession Number: NM_006268). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Apoptosised HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110064(DPF2 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:110064(DPF2 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using DPF2 antibody Catalog No:110064 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-DPF2 (IP:Catalog No:110064, 4ug; Detection:Catalog No:110064 1:500) with Jurkat cells lysate 1200ug.
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Background
DPF2 represents D4, zinc and double PHD fingers family 2
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References
- Liu C, Zhang D, Shen Y. DPF2 regulates OCT4 protein level and nuclear distribution. Biochimica et biophysica acta. 1853(12):3279-93. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"