-
Product Name
DOT1L Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to DOT1L
-
Tested applications
WB
-
Species reactivity
Human, Mouse, Rat
-
Alternative names
DOT1L antibody; DOT1 antibody; KMT4 antibody; DOT1 like histone lysine methyltransferase antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 250-500 of human DOT1L (NP_115871.1).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
-
Validations
Western blot - DOT1L Polyclonal Antibody
Western blot analysis of extracts of HeLa cells, using DOT1L antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
-
Background
Histone methyltransferase. Methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. Binds to DNA.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"