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Dopamine beta Hydroxylase antibody

Cat.#: 110060

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Product Information

  • Product Name
    Dopamine beta Hydroxylase antibody
  • Documents
  • Description
    Dopamine beta Hydroxylase Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells, HeLa cells, SH-SY5Y cells. Positive IHC detected in human liver cancer tissue. Positive IF detected in HepG2 cells, HeLa cells. Observed molecular weight by Western-blot: 63 kDa
  • Tested applications
    ELISA, WB, IF, IHC
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    DBH antibody; Dopamine beta Hydroxylase antibody; Dopamine beta monooxygenase antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Dopamine beta Hydroxylase recombinant protein (Accession Number: BC017174). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IHC: 1:50-1:500

    IF: 1:10-1:100

  • Validations

    HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:110060(DBH antibody) at dilution of 1:1000

    HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:110060(DBH antibody) at dilution of 1:1000

    Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using Catalog No:110060(DBH Antibody) at dilution of 1:100 (under 40x lens).

    Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using Catalog No:110060(DBH Antibody) at dilution of 1:100 (under 40x lens).

    Immunofluorescent analysis of HepG2 cells using Catalog No:110060(DBH Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of HepG2 cells using Catalog No:110060(DBH Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    DBH(Dopamine beta-hydroxylase) belongs to the copper type II ascorbate-dependent monooxygenase family and catalyzes the oxidative hydroxylation of dopamine to norepinephrine. It is almost exclusively located in the adrenal medulla and the synaptic vesicles of postganglionic sympathetic neurons(PMID:11857564).The DBH is glycosylated (63--67 kDa) while still in the membrane(PMID:2325165) and it can form a dimer(PMID:8546710). Defects in DBH are the cause of dopamine beta-hydroxylase deficiency (DBH deficiency).

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