Dopamine beta Hydroxylase antibody
Dopamine beta Hydroxylase Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells, HeLa cells, SH-SY5Y cells. Positive IHC detected in human liver cancer tissue. Positive IF detected in HepG2 cells, HeLa cells. Observed molecular weight by Western-blot: 63 kDa
ELISA, WB, IF, IHC
Human; other species not tested.
DBH antibody; Dopamine beta Hydroxylase antibody; Dopamine beta monooxygenase antibody
This antibody was obtained by immunization of Dopamine beta Hydroxylase recombinant protein (Accession Number: BC017174). Purification method: Antigen affinity purified.
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Store at -20℃. DO NOT ALIQUOT
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:110060(DBH antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using Catalog No:110060(DBH Antibody) at dilution of 1:100 (under 40x lens).
Immunofluorescent analysis of HepG2 cells using Catalog No:110060(DBH Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
DBH(Dopamine beta-hydroxylase) belongs to the copper type II ascorbate-dependent monooxygenase family and catalyzes the oxidative hydroxylation of dopamine to norepinephrine. It is almost exclusively located in the adrenal medulla and the synaptic vesicles of postganglionic sympathetic neurons(PMID:11857564).The DBH is glycosylated (63--67 kDa) while still in the membrane(PMID:2325165) and it can form a dimer(PMID:8546710). Defects in DBH are the cause of dopamine beta-hydroxylase deficiency (DBH deficiency).
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