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Product Name
DOK1 Polyclonal Antibody
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Description
Polyclonal antibody to DOK1
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
DOK1 antibody; P62DOK antibody; pp62 antibody; docking protein 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human DOK1 (NP_001372.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100 -
Validations
Western blot - DOK1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using DOK1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - DOK1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human breast cancer using DOK1 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - DOK1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using DOK1 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - DOK1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human breast using DOK1 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - DOK1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human gastric cancer using DOK1 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - DOK1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse heart using DOK1 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - DOK1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse pancreas using DOK1 Antibody at dilution of 1:100 (40x lens).
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Background
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"