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Product Name
DOCK8 Polyclonal Antibody
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Description
Polyclonal antibody to DOCK8
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Tested applications
WB
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Species reactivity
Human
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Alternative names
DOCK8 antibody; HEL-205 antibody; MRD2 antibody; ZIR8 antibody; dedicator of cytokinesis 8 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic Peptide of human DOCK8
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, pH7.3.
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Storage instructions
Store at 4℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
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Validations
Western blot - DOCK8 Polyclonal Antibody
Western blot analysis of extracts of THP-1 cells, using DOCK8 antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
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Background
Guanine nucleotide exchange factor (GEF) which specifically activates small GTPase CDC42 by exchanging bound GDP for free GTP. During immune responses, required for interstitial dendritic cell (DC) migration by locally activating CDC42 at the leading edge membrane of DC (By similarity). Required for CD4(+) T-cell migration in response to chemokine stimulation by promoting CDC42 activation at T cell leading edge membrane. Is involved in NK cell cytotoxicity by controlling polarization of microtubule-organizing center (MTOC), and possibly regulating CCDC88B-mediated lytic granule transport to MTOC during cell killing.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"