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Product Name
DOCK2 Polyclonal Antibody
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Description
Polyclonal antibody to DOCK2
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Tested applications
WB
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Species reactivity
Human, Mouse
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Alternative names
DOCK2 antibody; IMD40 antibody; dedicator of cytokinesis 2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1551-1830 of human DOCK2 (NP_004937.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
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Validations
Western blot - DOCK2 Polyclonal Antibody
Western blot analysis of extracts of Jurkat cells, using DOCK2 antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
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Background
Involved in cytoskeletal rearrangements required for lymphocyte migration in response of chemokines. Activates RAC1 and RAC2, but not CDC42, by functioning as a guanine nucleotide exchange factor (GEF), which exchanges bound GDP for free GTP. May also participate in IL2 transcriptional activation via the activation of RAC2.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"