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Product Name
DOCK180-Specific antibody
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Description
DOCK180-Specific Rabbit Polyclonal antibody. Positive IF detected in Hela cells. Positive WB detected in HeLa cells, human placenta tissue. Observed molecular weight by Western-blot: 180kd
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Tested applications
ELISA, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ced5 antibody; dedicator of cytokinesis 1 antibody; DOCK1 antibody; DOCK180 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_001380). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110042(DocK1-Specific antibody) at dilution of 1:400
Immunofluorescent analysis of Hela cells, using DocK1 antibody Catalog No:110042 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
DocK1, also named as DocK180, belongs to the DocK family. It is involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. DocK1 functions as a guanine nucleotide exchange factor (GEF), which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. Its GEF activity may be enhanced by ELMO1. The antibody is specific to DocK1.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"