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Product Name
DOCK180 antibody
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Description
DOCK180 Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells, A431 cells, HeLa cells, MCF7 cells. Positive IP detected in HeLa cells. Positive IHC detected in human colon tissue. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 180 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human; other species not tested.
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Alternative names
ced5 antibody; dedicator of cytokinesis 1 antibody; DOCK1 antibody; DOCK180 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of D℃K180 recombinant protein (Accession Number: NM_001380). Purification method: Antigen Affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:109976(DocK1 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human colon slide using Catalog No:109976(DocK1 Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human colon slide using Catalog No:109976(DocK1 Antibody) at dilution of 1:50
Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:109976(DocK1 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
IP Result of anti-DocK1 (IP:Catalog No:109976, 4ug; Detection:Catalog No:109976 1:1000) with HeLa cells lysate 3200ug.
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Background
DocK1 represents dedicator of cytokinesis 1
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