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Product Name
DOC2B antibody
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Description
DOC2B Rabbit Polyclonal antibody. Positive IF detected in HepG2. Positive WB detected in mouse kidney tissue, human brain tissue, mouse brain tissue, human testis tissue. Observed molecular weight by Western-blot: 46kd
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Tested applications
ELISA, WB, IF
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Species reactivity
human, mouse, rat; other species not tested.
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Alternative names
DOC2B antibody; DOC2BL antibody; double C2 like domains antibody; beta antibody; DOC2 beta antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_003585). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IF: 1:10-1:100
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Validations
mouse kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:109974(Doc2B antibody) at dilution of 1:600
Immunofluorescent analysis of HepG2 cells, using Doc2B antibody Catalog No:109974 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
Doc2B may be involved in constitutive membrane trafficking including dynein-dependent intracellular vesicle transport. In vitro, binds calcium and phospholipids. The antibody is specific to Doc2B.
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References
- Kabekkodu SP, Bhat S, Radhakrishnan R. DNA promoter methylation-dependent transcription of the double C2-like domain β (DOC2B) gene regulates tumor growth in human cervical cancer. The Journal of biological chemistry. 289(15):10637-49. 2014.
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