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Product Name
DNPEP antibody
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Description
DNPEP Rabbit Polyclonal antibody. Positive IP detected in HEK-293 cells. Positive WB detected in HEK-293 cells, human spleen tissue. Positive IF detected in MCF-7 cells. Observed molecular weight by Western-blot: 55 kDa
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Tested applications
ELISA, WB, IP, IF
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Species reactivity
Human; other species not tested.
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Alternative names
aspartyl aminopeptidase antibody; ASPEP antibody; DAP antibody; DNPEP antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DNPEP recombinant protein (Accession Number: NM_001319118). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IF: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:109971(DNPEP antibody) at dilution of 1:500
IP Result of anti-DNPEP (IP:Catalog No:109971, 3ug; Detection:Catalog No:109971 1:500) with HEK-293 cells lysate 1760ug.
Immunofluorescent analysis of MCF-7 cells using Catalog No:109971(DNPEP Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
DNPEP, also named as ASPEP and DAP, plays an important role in intracellular protein and peptide metabolism. It catalyzes the hydrolysis of N-terminal aspartyl and glutamyl residues.
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References
- Nakamura Y, Inloes JB, Katagiri T, Kobayashi T. Chondrocyte-specific microRNA-140 regulates endochondral bone development and targets Dnpep to modulate bone morphogenetic protein signaling. Molecular and cellular biology. 31(14):3019-28. 2011.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"