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Product Name
DNM3 antibody
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Description
DNM3 Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in mouse brain tissue. Positive IHC detected in human gliomas tissue. Observed molecular weight by Western-blot: 97kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DNM3 antibody; dynamin 3 antibody; Dynamin antibody; testicular antibody; KIAA0820 antibody; T dynamin antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DNM3 recombinant protein (Accession Number: NM_001278252). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:109964(DNM3 antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:109964(DNM3 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:109964(DNM3 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-DNM3 (IP:Catalog No:109964, 4ug; Detection:Catalog No:109964 1:500) with mouse brain tissue lysate 2640ug.
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Background
DNM3 (dynamin 3) is a member of dynamin proteins that participate in membrane rearrangements such as vesicular trafficking and phaocytosis. Three dynamin proteins encoded by different genes are present in mammals: DNM1 is mainly expressed in central nervous system, DNM2 ubiquitously expressed, and DNM3 expressed chiefly in the testis and brain. Western blot using this antibody specifically detected the 100 kDa DNM3 in mouse brain. It doesn't cross-react with other DNMs. (22977254, 21671749)
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References
- Marino N, Collins JW, Shen C. Identification and validation of genes with expression patterns inverse to multiple metastasis suppressor genes in breast cancer cell lines. Clinical & experimental metastasis. 31(7):771-86. 2014.
- Reid AT, Lord T, Stanger SJ. Dynamin regulates specific membrane fusion events necessary for acrosomal exocytosis in mouse spermatozoa. The Journal of biological chemistry. 287(45):37659-72. 2012.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"