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Product Name
DNAJC7 antibody
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Description
DNAJC7 Rabbit Polyclonal antibody. Positive WB detected in Jurkat cells, HEK-293 cells, mouse testis tissue. Positive IF detected in HepG2 cells. Positive IHC detected in human pancreas cancer tissue. Positive FC detected in HepG2 cells. Observed molecular weight by Western-blot: 56 kDa
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Tested applications
ELISA, WB, IHC, IF, FC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
DANJC7 antibody; DJ11 antibody; DNAJC7 antibody; TPR repeat protein 2 antibody; TPR2 antibody; TTC2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DNAJC7 recombinant protein (Accession Number: NM_003315). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:109952(DNAJC7 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:109952(DNAJC7 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using DNAJC7 antibody Catalog No:109952 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
1X10^6 HepG2 cells were stained with 0.2ug DNAJC7 antibody (Catalog No:109952, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
DNAJC7 represents DnaJ (Hsp40) homolog, subfamily C, member 7
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References
- Kubo N, Wu D, Yoshihara Y, Sang M, Nakagawara A, Ozaki T. Co-chaperon DnaJC7/TPR2 enhances p53 stability and activity through blocking the complex formation between p53 and MDM2. Biochemical and biophysical research communications. 430(3):1034-9. 2013.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"