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Product Name
DNAJC19 antibody
- Documents
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Description
DNAJC19 Rabbit Polyclonal antibody. Positive IP detected in mouse heart tissue. Positive WB detected in HeLa cells, human brain tissue, human lung tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 13 kDa
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Tested applications
ELISA, IF, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DNAJC19 antibody; TIM14 antibody; TIMM14 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DNAJC19 recombinant protein (Accession Number: NM_145261). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IF: 1:10-1:100
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Validations
Immunofluorescent analysis of HepG2 cells, using DNAJC19 antibody Catalog No:110107 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110107(DNAJC19 Antibody) at dilution of 1:1000
IP Result of anti-DNAJC19 (IP:Catalog No:110107, 4ug; Detection:Catalog No:110107 1:1000) with mouse heart tissue lysate 4000ug.
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Background
DNAJC19 is a mitochondrial cochaperone that interacts with HSP70 chaperones through its conserved J-domain. As a transmembrane protein, DNAJC19 is strongly associated with the inner mitochondrial membrane. Mutations in DNAJC19 cause dilated cardiomyopathy with ataxia (DCMA). Recently DNAJC19 has been reported as interactor of PHB complex to regulate cardiolipin remodeling, which addressed the link of DNAJC19 to cardiomyopathy. This antibody is specific to DNAJC19 and had been tested by siRNA.(24856930)
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References
- Richter-Dennerlein R, Korwitz A, Haag M. DNAJC19, a mitochondrial cochaperone associated with cardiomyopathy, forms a complex with prohibitins to regulate cardiolipin remodeling. Cell metabolism. 20(1):158-71. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"