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Product Name
DNAJC10 antibody
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Description
DNAJC10 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, mouse liver tissue. Positive IHC detected in human pancreas cancer. Observed molecular weight by Western-blot: 75-80kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
human, mouse, rat; other species not tested.
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Alternative names
DNAJC10 antibody; DKFZp434J1813 antibody; MTHr antibody; Macrothioredoxin antibody; ER resident protein ERdj5 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DNAJC10 recombinant protein (Accession Number: BC034713). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110102(DNAJC10 antibody) at dilution of 1:1500
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:110102(DNAJC10 antibody) at dilution of 1:100 (under 10x lens)
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Background
DNAJC10(DnaJ homolog subfamily C member 10)is also named as ERDJ5. The human ER-resident protein (ERdj5) ubiquitously expresses and is abundant in secretory tissues such as pancreas and testis and it may be involved in assisting protein folding and quality control in the ER(PMID:12411443). The deduced 793-amino acid protein has a calculated molecular mass of about 91 kD. ERDJ5 contains an N-terminal hydrophobic sequence, followed by a type III DnaJ domain, 4 thioredoxin-like domains, and a C-terminal KDEL endoplasmic reticulum (ER) retention signal. It can be modified by N-linked glycosylation(PMID:12446677). This antibody is specific to DNAJC10.
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References
- Williams JM, Inoue T, Banks L, Tsai B. The ERdj5-Sel1L complex facilitates cholera toxin retrotranslocation. Molecular biology of the cell. 24(6):785-95. 2013.
- Walczak CP, Ravindran MS, Inoue T, Tsai B. A cytosolic chaperone complexes with dynamic membrane J-proteins and mobilizes a nonenveloped virus out of the endoplasmic reticulum. PLoS pathogens. 10(3):e1004007. 2014.
- Diamanti E, Mathieu S, Jeanneau C. Endoplasmic reticulum stress and mineralization inhibition mechanism by the resinous monomer HEMA. International endodontic journal. 46(2):160-8. 2013.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"