Product Name

DNAJB6 antibody

Documents

Description

DNAJB6 Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue. Positive IF detected in HepG2 cells. Positive WB detected in mouse brain tissue, HEK-293 cells, Y79 cells. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 27kd

Tested applications

ELISA, IHC, IF, WB, IP

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

DJ4 antibody; DKFZp566D0824 antibody; DnaJ antibody; DNAJB6 antibody; FLJ42837 antibody; Heat shOCk protein J2 antibody; HHDJ1 antibody; HSJ 2 antibody; HSJ2 antibody; MRJ antibody; MSJ 1 antibody; MSJ1 antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of DNAJB6 recombinant protein (Accession Number: NM_005494). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:200-1:2000

IP: 1:200-1:2000

IHC: 1:20-1:200

IF: 1:10-1:100

Validations

Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:110097(DNAJB6 antibody) at dilution of 1:100 (under 10x lens)

Immunofluorescent analysis of HepG2 cells, using DNAJB6 antibody Catalog No:110097 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:110097(DNAJB6 Antibody) at dilution of 1:600

IP Result of anti-DNAJB6 (IP:Catalog No:110097, 4ug; Detection:Catalog No:110097 1:800) with mouse brain tissue lysate 4000ug.

Background

DNAJB6 represents DnaJ (Hsp40) homolog, subfamily B, member 6

References

Zhang TT, Jiang YY, Shang L. Overexpression of DNAJB6 promotes colorectal cancer cell invasion through an IQGAP1/ERK-dependent signaling pathway. Molecular carcinogenesis. 54(10):1205-13. 2015.

DNAJB6 antibody

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