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Product Name
DNAJB3 antibody
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Description
DNAJB3 Rabbit Polyclonal antibody. Positive IHC detected in human testis. Positive WB detected in mouse testis tissue, Jurkat cells, PC-3 cells. Observed molecular weight by Western-blot: 28-30kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
human, mouse, rat; other species not tested.
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Alternative names
DNAJB3 antibody; HCG3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DNAJB3 recombinant protein (Accession Number: NM_001001394). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
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Validations
mouse testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:110094(DNAJB3 antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human testis using Catalog No:110094(DNAJB3 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human testis using Catalog No:110094(DNAJB3 antibody) at dilution of 1:100 (under 40x lens)
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Background
DNAJB3 represents DnaJ (Hsp40) homolog, subfamily B, member 3
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References
- Abubaker J, Tiss A, Abu-Farha M. DNAJB3/HSP-40 cochaperone is downregulated in obese humans and is restored by physical exercise. PloS one. 8(7):e69217. 2013.
- Tiss A, Khadir A, Abubaker J. Immunohistochemical profiling of the heat shock response in obese non-diabetic subjects revealed impaired expression of heat shock proteins in the adipose tissue. Lipids in health and disease. 13:106. 2014.
- Mitsugi R, Itoh T, Fujiwara R. Expression of Human DNAJ (Heat Shock Protein-40) B3 in Humanized UDP-glucuronosyltransferase 1 Mice. International journal of molecular sciences. 16(7):14997-5008. 2015.
- Abu-Farha M, Cherian P, Al-Khairi I. DNAJB3/HSP-40 cochaperone improves insulin signaling and enhances glucose uptake in vitro through JNK repression. Scientific reports. 5:14448. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"