Product Name

DNAJB11 antibody

Documents

Description

DNAJB11 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human ovary tumor tissue. Positive WB detected in HeLa cells, HepG2 cells, mouse heart tissue, rat liver tissue. Positive IP detected in mouse heart tissue. Observed molecular weight by Western-blot: 40kd

Tested applications

ELISA, WB, IHC, IF, IP

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

ABBP 2 antibody; ABBP2 antibody; APOBEC1 binding protein 2 antibody; DJ9 antibody; DnaJ protein homolog 9 antibody; DNAJB11 antibody; EDJ antibody; ER assOCiated DNAJ antibody; ER assOCiated dnaJ protein 3 antibody; ERdj3 antibody; ERj3 antibody; ERj3p antibody; hDj 9 antibody; hDj9 antibody; HEDJ antibody; Human DnaJ protein 9 antibody; PRO1080 antibody; PWP1 interacting protein 4 antibody; UNQ537 antibody; UNQ537/PRO1080 antibody

Immunogen

Human DNAJB11 Recombinant protein (GST tag)

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of DNAJB11 recombinant protein (Accession Number: NM_016306). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IP: 1:200-1:2000

IHC: 1:20-1:200

IF: 1:10-1:100

Validations

HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110089(DNAJB11 antibody) at dilution of 1:500

Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:110089(DNAJB11 antibody) at dilution of 1:100 (under 40x lens)

Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:110089(DNAJB11 antibody) at dilution of 1:100 (under 10x lens)

Immunofluorescent analysis of HepG2 cells, using DNAJB11 antibody Catalog No:110089 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

IP Result of anti-DNAJB11 (IP:Catalog No:110089, 4ug; Detection:Catalog No:110089 1:500) with mouse heart tissue lysate 3200ug.

Background

DNAJB11 represents DnaJ (Hsp40) homolog, subfamily B, member 11

References

Genereux JC, Qu S, Zhou M. Unfolded protein response-induced ERdj3 secretion links ER stress to extracellular proteostasis. The EMBO journal. 34(1):4-19. 2015.
Taguwa S, Maringer K, Li X. Defining Hsp70 Subnetworks in Dengue Virus Replication Reveals Key Vulnerability in Flavivirus Infection. Cell. 163(5):1108-23. 2015.
Hulleman JD, Brown SJ, Rosen H, Kelly JW. A high-throughput cell-based Gaussia luciferase reporter assay for identifying modulators of fibulin-3 secretion. Journal of biomolecular screening. 18(6):647-58. 2013.

DNAJB11 antibody

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