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Product Name
DNAJB1 antibody
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Description
DNAJB1 Rabbit Polyclonal antibody. Positive IHC detected in human lymphoma tissue. Positive IF detected in Hela cells. Positive IP detected in mouse lung tissue. Positive WB detected in mouse spleen tissue, COLO 320 cells, HEK-293 cells, HeLa cells, human liver tissue, mouse lung tissue. Observed molecular weight by Western-blot: 40kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DnaJ protein homolog 1 antibody; DNAJ1 antibody; DNAJB1 antibody; hDj 1 antibody; Hdj1 antibody; Heat shOCk 40 kDa protein 1 antibody; Heat shOCk protein 40 antibody; Hsp40 antibody; HSPF1 antibody; Human DnaJ protein 1 antibody; Sis1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DNAJB1 recombinant protein (Accession Number: NM_006145). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse spleen tissue were subjected to SDS PAGE followed by western blot with Catalog No:110088(DNAJB1 antibody) at dilution of 1:600
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:110088(DNAJB1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:110088(DNAJB1 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of Hela cells, using DNAJB1 antibody Catalog No:110088 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-DNAJB1 (IP:Catalog No:110088, 4ug; Detection:Catalog No:110088 1:500) with mouse lung tissue lysate 4000ug.
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Background
DNAJB1 represents DnaJ (Hsp40) homolog, subfamily B, member 1
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References
- Huang L, Yu Z, Zhang T, Zhao X, Huang G. HSP40 interacts with pyruvate kinase M2 and regulates glycolysis and cell proliferation in tumor cells. PloS one. 9(3):e92949. 2014.
- Yang L, Wang Y, Zhang Q. Identification of Hsf1 as a novel androgen receptor-regulated gene in mouse Sertoli cells. Molecular reproduction and development. 81(6):514-23. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"