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Product Name
DNAJA3 Polyclonal Antibody
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Description
Polyclonal antibody to DNAJA3
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
DNAJA3 antibody; HCA57 antibody; TID1 antibody; hTID-1 antibody; dnaJ homolog subfamily A member 3, mitochondrial antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 221-480 of human DNAJA3 (NP_005138.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - DNAJA3 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using DNAJA3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
Immunohistochemistry - DNAJA3 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using DNAJA3 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - DNAJA3 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat kidney using DNAJA3 Antibody at dilution of 1:100 (40x lens).
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Background
Modulates apoptotic signal transduction or effector structures within the mitochondrial matrix. Affect cytochrome C release from the mitochondria and caspase 3 activation, but not caspase 8 activation. Isoform 1 increases apoptosis triggered by both TNF and the DNA-damaging agent mytomycin C; in sharp contrast, isoform 2 suppresses apoptosis. Can modulate IFN-gamma-mediated transcriptional activity. Isoform 2 may play a role in neuromuscular junction development as an effector of the MUSK signaling pathway.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"