Human MSH2/hMSH2 (His & GST Tag) recombinant protein

Human MSH2/hMSH2 (His & GST Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.

DNA mismatch repair protein Msh2

COCA1; HNPCC1; LCFS2; AI788990; HNPCC; FCC1

P43246

MSH2

Baculovirus-Insect Cells

A DNA sequence encoding the human MSH2 (NP_000242.1) (Met1-Thr934) was expressed with a C-terminal polyhistidine-tagged GST tag at the N-terminus (his-GST).

Human

The recombinant human MSH2 consists 1171 amino acids and predicts a molecular mass of 132.6 kDa.

> 90 % as determined by SDS-PAGE.