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Anti-MSH2 (1B3) Mouse antibody

Cat.#: 168361

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Product Information

  • Product Name
    Anti-MSH2 (1B3) Mouse antibody
  • Documents
  • Description
    MSH2 (1B3) Mouse monoclonal antibody
  • Tested applications
    WB, IHC-P, ICC/IF, IP
  • Species reactivity
    Human, Monkey
  • Isotype
    Mouse IgG1
  • Preparation
    Antigen: Purified recombinant fragment of human MSH2 expressed in E. Coli.
  • Clonality
    Monoclonal
  • Formulation
    Ascitic fluid containing 0.03% sodium azide.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/500 - 1/2000

    IHC: 1/200 - 1/1000

    ICC: 1/200 - 1/1000

    ELISA: 1/10000

  • Validations

    Immunoprecipitation analysis of Hela cell lysates using MSH2 mouse mAb.

    Immunoprecipitation analysis of Hela cell lysates using MSH2 mouse mAb.

    Immunocytochemistry staining of HeLa cells using anti-MSH2 antibody (dilution 1:100).

    Immunocytochemistry staining of HeLa cells using anti-MSH2 antibody (dilution 1:100).

    Western blot detection of MSH2 in Hela,A431,A549 and 293T cell lysates using MSH2 mouse mAb (1:500 diluted).Predicted band size:100KDa.Observed band size:100KDa.

    Western blot detection of MSH2 in Hela,A431,A549 and 293T cell lysates using MSH2 mouse mAb (1:500 diluted).Predicted band size:100KDa.Observed band size:100KDa.

    Immunohistochemical analysis of paraffin-embedded human breast cancer (left) and lung cancer (right) tissues using MSH2 mouse mAb with DAB staining, showing nuclear localization.

    Immunohistochemical analysis of paraffin-embedded human breast cancer (left) and lung cancer (right) tissues using MSH2 mouse mAb with DAB staining, showing nuclear localization.

  • Background
    Swiss-Prot Acc.P43246.MSH2 is a 100 kDa nuclear antigen and encodes a protein of 934 amino acids. The MSH2 gene is one of 4 known genes encoding proteins involved in the repair of mismatch nucleotides following DNA replication or repair. Mutations in the MSH2 gene contribute to the development of sporadic colorectal carcinoma. MSHS mutations are responsible for 50% of inherited non-polyposis colorectal (HNPCC). The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related to human carcinogenesis. MSH-2 is involved in the initial cognition of mismatch nucleotides during the replication mismatch repair process.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"