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Product Name
DNA Ligase I antibody
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Description
DNA Ligase I Rabbit Polyclonal antibody. Positive IHC detected in human spleen tissue, human brain tissue, human placenta tissue, human testis tissue. Positive WB detected in HepG2 cells, A431 cells, HeLa cells, Jurkat cells. Positive IP detected in HepG2 cells. Observed molecular weight by Western-blot: 130 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DNA ligase 1 antibody; DNA Ligase I antibody; LIG1 antibody; ligase I antibody; DNA antibody; ATP dependent antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DNA Ligase I recombinant protein (Accession Number: NM_000234). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:110077(LIG1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human spleen using Catalog No:110077(LIG1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human spleen using Catalog No:110077(LIG1 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-LIG1 (IP:Catalog No:110077, 4ug; Detection:Catalog No:110077 1:500) with HepG2 cells lysate 3200ug.
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Background
LIG1, also named as LRIG1, acts as a feedback negative regulator of signaling by receptor tyrosine kinases, through a mechanism that involves enhancement of receptor ubiquitination and accelerated intracellular degradation. Read more about this antibody on the blog: http://blog.ptglab.com/index.php/proteintechs-anti-neil3-antibody-used-investigate-mechanisms-of-dna-repair/?preview=true
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References
- Han L, Masani S, Hsieh CL, Yu K. DNA ligase I is not essential for mammalian cell viability. Cell reports. 7(2):316-20. 2014.
- Masani S, Han L, Meek K, Yu K. Redundant function of DNA ligase 1 and 3 in alternative end-joining during immunoglobulin class switch recombination. Proceedings of the National Academy of Sciences of the United States of America. 113(5):1261-6. 2016.
- Lu G, Duan J, Shu S. Ligase I and ligase III mediate the DNA double-strand break ligation in alternative end-joining. Proceedings of the National Academy of Sciences of the United States of America. 113(5):1256-60. 2016.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"