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Product Name
DMD Polyclonal Antibody
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Description
Polyclonal antibody to DMD
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
DMD antibody; BMD antibody; CMD3B antibody; DXS142 antibody; DXS164 antibody; DXS206 antibody; DXS230 antibody; DXS239 antibody; DXS268 antibody; DXS269 antibody; DXS270 antibody; DXS272 antibody; MRX85 antibody; dystrophin antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 346-635 of human DMD (NP_004007.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:200 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - DMD Polyclonal Antibody
Western blot analysis of extracts of mouse heart, using DMD antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 90s.
Immunohistochemistry - DMD Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat heart using DMD antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - DMD Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse heart using DMD antibody at dilution of 1:100 (40x lens).
Immunofluorescence - DMD Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using DMD antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Anchors the extracellular matrix to the cytoskeleton via F-actin. Ligand for dystroglycan. Component of the dystrophin-associated glycoprotein complex which accumulates at the neuromuscular junction (NMJ) and at a variety of synapses in the peripheral and central nervous systems and has a structural function in stabilizing the sarcolemma. Also implicated in signaling events and synaptic transmission.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"