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Product Name
Mertansine (DM1) Monoclonal Antibody
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Description
Product has been tested in direct ELISA.
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Tested applications
ELISA
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Species reactivity
Chemical
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Isotype
Mouse IgG2b, κ
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Preparation
DM1 conjugated with BSA, Protein G affinity purified
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Clonality
Monoclonal
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Formulation
PBS(pH7.4), lyophilized. Reconstitution: Adding sterile water, prepare a stock solution of 2.0 mg/ml.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
ELISA: 1:5,000-1:20,000
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Validations
ELISA Binding Assay of Anti-DM1 Antibody to different proteins.
ELISA analysis of Anti-DM1 Monoclonal Antibody with DM1-BSA, DM1-KLH, BSA, KLH and Kadcyla. Anti-DM1 Monoclonal Antibody can bind to DM1-BSA, DM1-KLH or DM1 ADC but not carrier proteins.
ELISA Binding Assay of Anti-DM1 Antibody
Immobilized DM1 ADC at 1μg/mL (100μL/well) can bind anti-DM1 mouse monoclonal antibody.
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Background
Mertansine, also called DM1 or Maytansinoid DM1, is a microtubulin inhibitor and it inhibits the assembly of microtubules by binding to tubulin (at the rhizoxin binding site). Derivatives of maytansine are known as maytansinoids(e.g.: Mertansine(DM1), Ravtansine(DM4)). Mertansine can be attached to a monoclonal antibody with a linker to create an antibody-drug conjugate (ADC).
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References
- Lopus M, Oroudjev E, Wilson L, Wilhelm S, Widdison W, Chari R, Jordan MA. Maytansine and cellular metabolites of antibody-maytansinoid conjugates strongly suppress microtubule dynamics by binding to microtubules. Mol Cancer Ther. 2010 Oct;9(10):2689-99. doi: 10.1158/1535-7163.MCT-10-0644. PMID: 20937594; PMCID: PMC2954514.
- Zhong P, Qiu M, Zhang J, Sun H, Cheng R, Deng C, Meng F, Zhong Z. cRGD-installed docetaxel-loaded mertansine prodrug micelles: redox-triggered ratiometric dual drug release and targeted synergistic treatment of B16F10 melanoma. Nanotechnology. 2017 Jul 21;28(29):295103. doi: 10.1088/1361-6528/aa76cc. Epub 2017 Jun 2. PMID: 28574851.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"