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Product Name
DLGAP5 Polyclonal Antibody
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Description
Polyclonal antibody to DLGAP5
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Tested applications
WB, IF
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Species reactivity
Human, Mouse
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Alternative names
DLGAP5 antibody; DLG7 antibody; HURP antibody; DLG associated protein 5 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 547-846 of human DLGAP5 (NP_055565.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - DLGAP5 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using DLGAP5 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunofluorescence - DLGAP5 Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using DLGAP5 antibody . Blue: DAPI for nuclear staining.
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Background
Potential cell cycle regulator that may play a role in carcinogenesis of cancer cells. Mitotic phosphoprotein regulated by the ubiquitin-proteasome pathway. Key regulator of adherens junction integrity and differentiation that may be involved in CDH1-mediated adhesion and signaling in epithelial cells.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"