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Product Name
DKK1 antibody
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Description
DKK1 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human gliomas tissue. Positive WB detected in human placenta tissue, HeLa cells, K-562 cells, Sp2/0 cells. Observed molecular weight by Western-blot: 30-35 kDa
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Tested applications
ELISA, WB, IF, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Dickkopf 1 antibody; Dickkopf related protein 1 antibody; DKK 1 antibody; DKK1 antibody; hDkk 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DKK1 recombinant protein (Accession Number: NM_012242). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
human placenta tissue were subjected to SDS PAGE followed by western blot with Catalog No:109982(DKK1 antibody) at dilution of 1:500
Immunofluorescent analysis of HepG2 cells, using DKK1 antibody Catalog No:109982 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Immunohistochemistry of paraffin-embedded human gliomas tissue slide using Catalog No:109982(DKK1 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human gliomas tissue slide using Catalog No:109982(DKK1 Antibody) at dilution of 1:50 (under 40x lens)
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Background
DKK1, also named a SK and Dickkopf-1, belongs to the dickkopf family. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. SDS-PAGE and Western blot analysis demonstrated that DKK1 is expressed as a 35-kD doublet protein, which is larger than the deduced molecular mass of 26 kD. Sometime DKK1 is expressed as a 42- to 50-kD secreted protein, with little change observed after glycanase treatment.
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References
- Ying Q, Ansong E, Diamond AM, Lu Z, Yang W, Bie X. Quantitative proteomic analysis reveals that anti-cancer effects of selenium-binding protein 1 in vivo are associated with metabolic pathways. PloS one. 10(5):e0126285. 2015.
- Liu BL, Sun KX, Zong ZH, Chen S, Zhao Y. MicroRNA-372 inhibits endometrial carcinoma development by targeting the expression of the Ras homolog gene family member C (RhoC). Oncotarget. 2015.
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