DJK-5 peptide

DJK-5 peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-VQWRAIRVRVIR-NH2 (d-amino acids)

H-Val-Gln-Trp-Arg-Ala-Ile-Arg-Val-Arg-Val-Ile-Arg-NH2 (d-amino acids)

12

95.59%

C₇₀H₁₂₃N₂₇O₁₃

1550.89

Synthetic

Powder

DJK-5 (vqwrairvrvir; d-amino acids) and DJK-6 (vqwrrirvw-vir; d-amino acids) were identified from screening a library of 12-amino-acid-long peptides with the following design constraints: d-enantiomeric forms of only 9 amino acids (A, F, I, K, L, Q, R, V, W), 4 charged residues, 7–8 hydrophobic residues, and 0–1 Q residues. DJK-5 and DJK-6 were identified as being able to inhibit biofilm formation by several common bacterial strains, at concentrations below their MIC, and to eradicate established biofilms. Further, DJK-5 was shown to decrease abscess size and reduce bacterial burden in a mouse cutaneous abscess model induced by P. aeruginosa.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Mansour, S. C., Pletzer, D., de la Fuente-Núñez, C., Kim, P., Cheung, G. Y. C., Joo, H.-S., Otto, M., and Hancock, R. E. W. (2016) Bacterial Abscess Formation Is Controlled by the Stringent Stress Response and Can Be Targeted Therapeutically. EBioMedicine 12, 219−226.
• Kłodzińska, S. N., Pletzer, D., Rahanjam, N., Rades, T., Hancock, R. E. W., and Nielsen, H. M. (2019) Hyaluronic Acid-Based Nanogels Improve in vivo Compatibility of the Anti-Biofilm Peptide DJK-5. Nanomedicine 20, 102022.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.