Rat ADAM17 (His Tag) recombinant protein

Rat ADAM17 (His Tag) recombinant protein

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• Material Safety Data Sheets (MSDS)

Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface (By similarity). Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Acts as an activator of Notch pathway by mediating cleavage of Notch, generating the membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). Plays a role in the proteolytic processing of ACE2 (By similarity).

Disintegrin and metalloproteinase domain-containing protein 17

ADAM METALLOPEPTIDASE DOMAIN 18; ADAM 17; ADAM18; ADAM METALLOPEPTIDASE DOMAIN 17; TNF-ALPHA CONVERTASE; SNAKE VENOM-LIKE PROTEASE; CSVP; A DISINTEGRIN AND METALLOPEPTIDASE DOMAIN 17; NISBD1; CONVERTING ENZYME; TUMOR NECROSIS FACTOR; A DISINTEGRIN AND METALLOPROTEASE DOMAIN 17; CD156B; ADAM17; TACE; TNF-ALPHA CONVERTING ENZYME; DISINTEGRIN AND METALLOPROTEINASE DOMAIN-CONTAINING PROTEIN 17; NISBD; ALPHA; A DISINTEGRIN AND METALLOPROTEINASE DOMAIN 17

Q9Z1K9

Adam17; Tace

Human Cells

A DNA sequence encoding the rat ADAM17 (Q9Z1K9) (Met1-Asp563) was expressed with a polyhistidine tag at the C-terminus.

Rat

The recombinant rat ADAM17 comprises 557 amino acids and predicts a molecular mass of 63 kDa. The apparent molecular mass of the recombinant protein is approximately 57 and 20 kDa in SDS-PAGE under reducing conditions due to glycosylation.

(73.2+23.7) % as determined by SDS-PAGE

Measured by its ability to cleave a fluorogenic peptide substrate Mca-PLAQAV-Dpa-RSSSR-NH2 (Catalog # ES003).
The specific activity is >250 pmol/min/µg.