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Product Name
ADAM17 Polyclonal Antibody
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Description
Polyclonal antibody to ADAM17
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
ADAM17 antibody; ADAM18 antibody; CD156B antibody; CSVP antibody; NISBD antibody; NISBD1 antibody; TACE antibody; ADAM metallopeptidase domain 17 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 700 to the C-terminus of human ADAM17 (NP_003174.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - ADAM17 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ADAM17 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 5s.
Immunohistochemistry - ADAM17 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat heart using ADAM17 antibody at dilution of 1:100 (40x lens).
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Background
Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Acts as an activator of Notch pathway by mediating cleavage of Notch, generating the membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). Plays a role in the proteolytic processing of ACE2. Plays a role in hemostasis through shedding of GP1BA, the platelet glycoprotein Ib alpha chain (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"