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Product Name
DISC1 Polyclonal Antibody
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Description
Polyclonal antibody to DISC1
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
DISC1 antibody; C1orf136 antibody; SCZD9 antibody; disrupted in schizophrenia 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human DISC1 (NP_001158009.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - DISC1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using DISC1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
Western blot - DISC1 Polyclonal Antibody
Western blot analysis of extracts of various cells, using DISC1 antibody at 1:1000 dilution ratio through one-step method.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:4000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3min.
Immunohistochemistry - DISC1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human stomach using DISC1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - DISC1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat kidney using DISC1 antibody at dilution of 1:100 (40x lens).
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Background
Involved in the regulation of multiple aspects of embryonic and adult neurogenesis. Required for neural progenitor proliferation in the ventrical/subventrical zone during embryonic brain development and in the adult dentate gyrus of the hippocampus. Participates in the Wnt-mediated neural progenitor proliferation as a positive regulator by modulating GSK3B activity and CTNNB1 abundance. Plays a role as a modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including neuron positioning, dendritic development and synapse formation. Inhibits the activation of AKT-mTOR signaling upon interaction with CCDC88A. Regulates the migration of early-born granule cell precursors toward the dentate gyrus during the hippocampal development. Plays a role, together with PCNT, in the microtubule network formation.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"