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Product Name
Human DPYS / Dihydropyrimidinase (His & GST Tag) recombinant protein
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Description
Catalyzes the second step of the reductive pyrimidine degradation, the reversible hydrolytic ring opening of dihydropyrimidines. Can catalyze the ring opening of 5,6-dihydrouracil to N-carbamyl-alanine and of 5,6-dihydrothymine to N-carbamyl-amino isobutyrate.
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Protein name
Dihydropyrimidinase
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Protein short names
DHP; DIHYDROPYRIMIDINE AMIDOHYDROLASE; DHPASE; DPYS; 1200017I10RIK; 1300004I01RIK; HYDANTOINASE; DIHYDROPYRIMIDINASE
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Uniprot ID
Q14117
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Gene Name
DPYS
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Source/Expression Host
Baculovirus-Insect Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the human DPYS (NP_001376.1) (Met1-Pro519) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
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Protein Species
Human
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Molecular weight
The recombinant human DPYS/GST chimera consists of 756 amino acids and has a calculated molecular mass of 84.4 kDa. The recombinant protein migrates as an approximately 69 kDa band in SDS-PAGE under reducing conditions.
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Purity
> 95 % as determined by SDS-PAGE
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Validations
Human DPYS / Dihydropyrimidinase Protein (His & GST Tag) SDS-PAGE
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