-
Product Name
DIDO1 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to DIDO1
-
Tested applications
WB, IF
-
Species reactivity
Human
-
Alternative names
DIDO1 antibody; BYE1 antibody; C20orf158 antibody; DATF-1 antibody; DATF1 antibody; DIDO2 antibody; DIDO3 antibody; DIO-1 antibody; DIO1 antibody; dJ885L7.8 antibody; death-inducer obliterator 1 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human DIDO1 (NP_071388.2).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IF 1:20 - 1:50 -
Validations
Western blot - DIDO1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using DIDO1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunofluorescence - DIDO1 Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using DIDO1 antibody . Blue: DAPI for nuclear staining.
-
Background
Putative transcription factor, weakly pro-apoptotic when overexpressed (By similarity). Tumor suppressor. Required for early embryonic stem cell development.; Isoform 2: Displaces isoform 4 at the onset of differentiation, required for repression of stemness genes.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"