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Product Name
DHX36 antibody
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Description
DHX36 Rabbit Polyclonal antibody. Positive IHC detected in human prostate tissue. Positive WB detected in human brain tissue, human liver tissue, PC-3 cells. Positive IP detected in PC-3 cells. Observed molecular weight by Western-blot: 120 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DDX36 antibody; DEAH box protein 36 antibody; DHX36 antibody; G4R1 antibody; KIAA1488 antibody; MLE like protein 1 antibody; MLEL1 antibody; RHAU antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DHX36 recombinant protein (Accession Number: BC036035). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:109932(DHX36 antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human prostate using Catalog No:109932(DHX36 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human prostate using Catalog No:109932(DHX36 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-DHX36 (IP:Catalog No:109932, 5ug; Detection:Catalog No:109932 1:500) with PC-3 cells lysate 1880ug.
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Background
DHX36, also known as RNA associated with AU-rich element (RHAU) and G4R1, is an adenosine triphosphate (ATP)-dependent RNA helicase that belongs to the DExH/D family of RNA modifying enzymes. Proteins in this family involve in a wide range of cellular functions including RNA splicing, ribosome assembly, messenger RNA (mRNA) stability, microRNA processing, ribonucleoprotein remodeling and RNA trafficking. DHX36 is an RNA helicase that has specificity for DNA and RNA G4-quadruplexes, and resolves G4 structures into separate nucleotide strands for metabolic processing of G-rich sequence.
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References
- Nie J, Jiang M, Zhang X. Post-transcriptional Regulation of Nkx2-5 by RHAU in Heart Development. Cell reports. 13(4):723-32. 2015.
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