-
Product Name
DHX15 antibody
- Documents
-
Description
DHX15 Rabbit Polyclonal antibody. Positive WB detected in NIH/3T3 cells, HEK-293 cells, HeLa cells. Positive IP detected in NIH/3T3 cells. Observed molecular weight by Western-blot: 91 kDa
-
Tested applications
ELISA, WB, IP
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
ATP dependent RNA helicase #46 antibody; DBP1 antibody; DDX15 antibody; DEAH box protein 15 antibody; DHX15 antibody; HRH2 antibody; PRP43 antibody; PRPF43 antibody; PrPp43p antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of DHX15 recombinant protein (Accession Number: NM_001358). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
-
Validations
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with Catalog No:109927(DHX15 antibody) at dilution of 1:300
IP Result of anti-DHX15 (IP:Catalog No:109927, 4ug; Detection:Catalog No:109927 1:600) with NIH/3T3 cells lysate 2000ug.
-
Background
DHX15, also named as DBP1 and DDX15, is a DExD/H-box protein that has been identified in spliceosome, through its carboxyl (C)-terminal G-patch domain and stimulates the helicase activity of DHX15. DHX15 is a Pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA. The MW of DHX15 is about 90-95kd.
-
References
- Niu Z, Jin W, Zhang L, Li X. Tumor suppressor RBM5 directly interacts with the DExD/H-box protein DHX15 and stimulates its helicase activity. FEBS letters. 586(7):977-83. 2012.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"