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Product Name
DHRS4 antibody
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Description
DHRS4 Rabbit Polyclonal antibody. Positive IHC detected in human cervix tissue. Positive WB detected in HepG2 cells, HeLa cells, mouse liver tissue. Positive IP detected in mouse liver tissue. Observed molecular weight by Western-blot: 29 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DHRS4 antibody; DHRS4L2 antibody; FLJ11008 antibody; humNRDR antibody; NRDR antibody; PHCR antibody; PSCD antibody; SCAD SRL antibody; SDR SRL antibody; SDR25C1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DHRS4 recombinant protein (Accession Number: XM_047446404). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:109925(DHRS4 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human cervix using Catalog No:109925(DHRS4 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human cervix using Catalog No:109925(DHRS4 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-DHRS4 (IP:Catalog No:109925, 4ug; Detection:Catalog No:109925 1:400) with mouse liver tissue lysate 4000ug.
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Background
DHRS4(Dehydrogenase/reductase SDR family member 4) is also named as PHCR , NRDR ,PSCD , SCAD-SRL, and belongs to the short-chain dehydrogenases/reductases (SDR) family. It can also catalyze the oxidation of all-trans-retinol with NADP as co-factor, but with much lower efficiency. Human DHRS4 is a tetramer composed of 27 kDa subunits, which is inactivated at low temperature without dissociation into subunits(PMID:18571493).
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