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Product Name
DHRS3 antibody
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Description
DHRS3 Rabbit Polyclonal antibody. Positive WB detected in A375 cells. Positive IP detected in A375 cells. Observed molecular weight by Western-blot: 34 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DD83.1 antibody; DHRS3 antibody; RDH17 antibody; retSDR1 antibody; Rsdr1 antibody; SDR1 antibody; SDR16C1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DHRS3 recombinant protein (Accession Number: NM_004753). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
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Validations
A375 cells were subjected to SDS PAGE followed by western blot with Catalog No:109924(DHRS3 antibody) at dilution of 1:500
IP Result of anti-DHRS3 (IP:Catalog No:109924, 4ug; Detection:Catalog No:109924 1:500) with A375 cells lysate 3600ug.
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Background
DHRS3(Short-chain dehydrogenase/reductase 3) is also named as retSDR1,DD83.1 and belongs to the short-chain dehydrogenases/reductases (SDR) family. It catalyzes the reduction of all-trans-retinal to all-trans-retinol in the presence of NADPH. It is detected high levels of expression in fetal kidney, liver, and lung and in adult heart, placenta, lung, liver, kidney, pancreas, thyroid, testis, stomach, trachea, spinal cord and lower levels in skeletal muscle, intestine, and lymph node. retSDR1 is barely detectable in adrenals, brain, thymus, and hematopoietic tissues(PMID:11861404). retSDR1 may play a more general role in retinol metabolism since its expression was observed in many fetal and adult tissues(PMID:20543567).
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References
- Lee SA, Belyaeva OV, Wu L, Kedishvili NY. Retinol dehydrogenase 10 but not retinol/sterol dehydrogenase(s) regulates the expression of retinoic acid-responsive genes in human transgenic skin raft culture. The Journal of biological chemistry. 286(15):13550-60. 2011.
- Deisenroth C, Itahana Y, Tollini L, Jin A, Zhang Y. p53-Inducible DHRS3 is an endoplasmic reticulum protein associated with lipid droplet accumulation. The Journal of biological chemistry. 286(32):28343-56. 2011.
- Kuroda K, Venkatakrishnan R, Salker MS. Induction of 11β-HSD 1 and activation of distinct mineralocorticoid receptor- and glucocorticoid receptor-dependent gene networks in decidualizing human endometrial stromal cells. Molecular endocrinology (Baltimore, Md.). 27(2):192-202. 2013.
- Billings SE, Pierzchalski K, Butler Tjaden NE. The retinaldehyde reductase DHRS3 is essential for preventing the formation of excess retinoic acid during embryonic development. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 27(12):4877-89. 2013.
- Kuroda K, Venkatakrishnan R, James S. Elevated periimplantation uterine natural killer cell density in human endometrium is associated with impaired corticosteroid signaling in decidualizing stromal cells. The Journal of clinical endocrinology and metabolism. 98(11):4429-37. 2013.
- Adams MK, Belyaeva OV, Wu L, Kedishvili NY. The retinaldehyde reductase activity of DHRS3 is reciprocally activated by retinol dehydrogenase 10 to control retinoid homeostasis. The Journal of biological chemistry. 289(21):14868-80. 2014.
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