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Product Name
DHCR7 Polyclonal Antibody
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Description
Polyclonal antibody to DHCR7
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse
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Alternative names
DHCR7 antibody; SLOS antibody; 7-dehydrocholesterol reductase antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human DHCR7
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, pH7.3.
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Storage instructions
Store at 4℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100 -
Validations
Western blot - DHCR7 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using DHCR7 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
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Background
Production of cholesterol by reduction of C7-C8 double bond of 7-dehydrocholesterol (7-DHC).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"