DGKE Polyclonal Antibody

Cat.#: 165515

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Product Information

  • Product Name
    DGKE Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to DGKE
  • Tested applications
    WB, IHC, IF
  • Species reactivity
    Human, Mouse
  • Alternative names
    DGKE antibody; AHUS7 antibody; DAGK5 antibody; DAGK6 antibody; DGK antibody; NPHS7 antibody; diacylglycerol kinase epsilon antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 40-260 of human DGKE (NP_003638.1).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IHC 1:50 - 1:200
    IF 1:50 - 1:200
  • Validations

    Western blot - DGKE Polyclonal Antibody

    Western blot - DGKE Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using DGKE antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.

    Immunohistochemistry - DGKE Polyclonal Antibody

    Immunohistochemistry - DGKE Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded human gastric using DGKE antibody at dilution of 1:100 (40x lens).

    Immunohistochemistry - DGKE Polyclonal Antibody

    Immunohistochemistry - DGKE Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded mouse kidney using DGKE antibody at dilution of 1:100 (40x lens).

    Immunofluorescence - DGKE Polyclonal Antibody

    Immunofluorescence - DGKE Polyclonal Antibody

    Immunofluorescence analysis of U2OS cells using DGKE antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

  • Background
    Highly selective for arachidonate-containing species of diacylglycerol (DAG). May terminate signals transmitted through arachidonoyl-DAG or may contribute to the synthesis of phospholipids with defined fatty acid composition.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"