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Product Name
DGKE antibody
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Description
DGKE Rabbit Polyclonal antibody. Positive IHC detected in human prostate cancer tissue. Positive WB detected in HEK-293 cells, human brain tissue. Observed molecular weight by Western-blot: 60-64kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DAG kinase epsilon antibody; DAGK5 antibody; DAGK6 antibody; DGK antibody; DGK epsilon antibody; DGKE antibody; Diacylglycerol kinase epsilon antibody; Diglyceride kinase epsilon antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DGKE recombinant protein (Accession Number: BC022297). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:109911(DGKE antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:109911(DGKE antibody) at dilution of 1:50 (under 10x lens)
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Background
DGKE(Diacylglycerol kinase epsilon) is also named as DAGK5 and belongs to the eukaryotic diacylglycerol kinase family.It may be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction(PMID:16689942).When expressed in mammalian cells, DGK-epsilon showed specificity for arachidonyl-containing diacylglycerol.
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