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Product Name
DGKD Polyclonal Antibody
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Description
Polyclonal antibody to DGKD
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Tested applications
WB
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Species reactivity
Human
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Alternative names
DGKD antibody; DGKdelta antibody; dgkd-2 antibody; diacylglycerol kinase delta antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 960-1140 of human DGKD (NP_690618.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - DGKD Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using DGKD antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.
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Background
May function as signaling molecule.; Isoform 2 may be involved in cell growth and tumorigenesis. Involved in clathrin-dependent endocytosis.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"