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Product Name
DGKA antibody
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Description
DGKA Rabbit Polyclonal antibody. Positive IHC detected in human gliomas tissue, human brain tissue. Positive IP detected in Jurkat cells. Positive WB detected in mouse cerebellum tissue, HEK-293 cells, HeLa cells, human cerebellum tissue, Jurkat cells. Observed molecular weight by Western-blot: 83kd
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Tested applications
ELISA, IHC, IP, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
80 kDa diacylglycerol kinase antibody; DAG kinase alpha antibody; DAGK antibody; DAGK1 antibody; DGK alpha antibody; DGKA antibody; Diacylglycerol kinase alpha antibody; Diglyceride kinase alpha antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DGKA recombinant protein (Accession Number: NM_201445). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
IP Result of anti-DGKA (IP:Catalog No:109909, 3ug; Detection:Catalog No:109909 1:1000) with Jurkat cells lysate 1500ug.
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:109909(DGKA antibody) at dilution of 1:100 (under 10x lens)
mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with Catalog No:109909(DGKA Antibody) at dilution of 1:1000
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Background
DGKA(Diacylglycerol kinase alpha) is also named as DAGK, DAGK1 and belongs to the eukaryotic diacylglycerol kinase family,which is suggested to attenuate diacylglycerol-induced cell responses through the phosphorylation of this second messenger to phosphatidic acid.DGKA regulates the secretion of lethal exosomes bearing Fas ligand during activation-induced cell death of T lymphocytes.
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References
- Dominguez CL, Floyd DH, Xiao A. Diacylglycerol kinase α is a critical signaling node and novel therapeutic target in glioblastoma and other cancers. Cancer discovery. 3(7):782-97. 2013.
- Kefas B, Floyd DH, Comeau L. A miR-297/hypoxia/DGK-α axis regulating glioblastoma survival. Neuro-oncology. 15(12):1652-63. 2013.
- Gellett AM, Kharel Y, Sunkara M, Morris AJ, Lynch KR. Biosynthesis of alkyl lysophosphatidic acid by diacylglycerol kinases. Biochemical and biophysical research communications. 422(4):758-63. 2012.
- Kawaguchi M, Valencia JC, Namiki T, Suzuki T, Hearing VJ. Diacylglycerol kinase regulates tyrosinase expression and function in human melanocytes. The Journal of investigative dermatology. 132(12):2791-9. 2012.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"