Cart 2

DGCR8 N-terminal antibody

Cat.#: 109906

Size:

Special Price 239.0 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    DGCR8 N-terminal antibody
  • Documents
  • Description
    DGCR8 N-terminal Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive WB detected in HeLa cells, A431 cells, HEK-293 cells, HepG2 cells, Jurkat cells. Positive IP detected in A431 cells. Observed molecular weight by Western-blot: 88-100 kDa
  • Tested applications
    ELISA, WB, IP, IF
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    C22orf12 antibody; DGCR8 antibody; DGCRK6 antibody; Gy1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of DGCR8 N-terminal recombinant protein (Accession Number: NM_001190326). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:200-1:2000

    IF: 1:20-1:200

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:109906(DGCR8 Antibody) at dilution of 1:600

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:109906(DGCR8 Antibody) at dilution of 1:600

    IP Result of anti-DGCR8 (IP:Catalog No:109906, 4ug; Detection:Catalog No:109906 1:600) with A431 cells lysate 2800ug.

    IP Result of anti-DGCR8 (IP:Catalog No:109906, 4ug; Detection:Catalog No:109906 1:600) with A431 cells lysate 2800ug.

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:109906(DGCR8 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:109906(DGCR8 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    DGCR8 is a RNA-binding protein that assists the Rnase III enzyme Drosha in the processing of microRNAs (miRNAs), which regulate the expression of a large number of protein-coding genes[PMID: 22580560]. DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains, may be an essential component of the primary miRNAs processing complex, along with Drosha, promoting the processing of primary microRNA to precursor microRNA. It is ubiquitous expressed in human and mouse tissues, and is deleted in DiGeorge syndrome[22323604]. The calculated molecular weight of DGCR8 is a 82-86 kDa protein, but the post-modified DGCR8 is about 120 kDa.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"